FIZIKA A 7 (1998) 1, 17-27

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EFFECTS OF HIGH-INTENSITY UV RADIATION ON ISOLATED AND DNA-INTERCALATED ETHIDIUM BROMIDE

E. KESKINOVAa, D. ANGELOVa, SV. ANGELOVAa, Z. SHAQIRIa and A. VESELIb

aInstitute of Solid State Physics, Bulgarian Academy of Sciences, Tzarigradsko Chaussee blvd.72, BG-1784, Sofia, Bulgaria
bFaculty of Natural and Mathematical Sciences, 39000 Prishtina, Yugoslavia

Received 28 August 1997; revised manuscript received 30 March 1998; Accepted 18 May 1998

High-intensity picosecond laser flash photolysis has been used for investigating ethidium bromide (EtBr). The quantum yield of primary photoproducts as a function of the laser intensity has been obtained. DNA intercalated EtBr and water solutions of EtBr have been investigated. The results have been compared. They show that two-photon processes are predominantly responsible for generation of hydrated electron (eaq-). The primary processes of DNA photosensitization have also been discussed.

PACS numbers: 87.15.Rn, 87.50.-a
UDC 535.217, 539.21

 

 

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